10 mL URINE from a well mixed 24 hr collection preserved (Boric Acid or HCL)
Instruct the patient to void at 8 AM and discard the specimen. Then collect all urine including the final specimen voided at the end of the 24 Hr. period (ie, 8 AM the next morning) in a plastic bottle containing 1 g boric acid per liter of urine. Mix well. Screw the lid on securely. Hall is also an acceptable preservative.