Test Code

• 6166

Test Synonym

• Pyruvic Acid (Pyruvate), Blood

Volume

• 0.5 mL

Minimum Volume

• 0.2 mL

Specimen Requirements

• Supernatant

Container

• Transfer Tube

Storage

• Refrigerated

Turn Around Time

• 5 days

CPT Codes

• 84210
  *The CPT codes provided are based on AMA guidelines and are provided for informational purposes only. CPT coding is the responsibility of the billing entity. Please direct any questions regarding the CPT coding to the payer being billed.

Special Instructions

• 0.5 mL Clear supernatant from oxalated WHOLE BLOOD

Additional Information

• Draw blood into chilled gray-top tube. Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). NOTE: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained. Shake mixture vigorously for about 30 seconds. Refrigerate for five minutes to ensure complete protein precipitation. Centrifuge 5 to 10 minutes at approximately 1500xg. Immediately transfer the CLEAR SUPERNATANT to a transport tube. Label the transport tube as SUPERNATANT and send to the laboratory. NOTE: A second centrifugation of the supernatant may be necessary to obtain a clear protein- free solution.

Specimen Stability

• AMBIENT: 5 days REFRIGERATED: 7 days FROZEN: 7 days